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Catalogue of recombinant enzymes for proteomic research

Class

Product Name

Action Sites

Application

Specification

(lyophilized powder)

 Sequencing Grade

Recombinant Lysine Endopeptidase

(Lys-C)

Specifical cleaving at the carboxyl side of Lysine Residue including Pro-Lys, Asp-Lys and other sequences hard to be identified

Proteomic research

Peptide mapping

Protein sequencing

Identification of phosphorylation

Analysis of disulfide bond

Analysis of N-glycan

20μg

20μg5 vials

Recombinant

Tripsin

(TP)

Specifical cleaving at the carboxyl side of Arg and Lys

Proteomic Research

Peptide mapping

Protein sequencing

 

20μg

20μg5 vials

RLys-C/RTP mix

Specifical cleaving at the carboxyl side of Arg and Lys

Peptide mapping, especially suitable for MS analysis with large molecular or high- hydrophobic proteins, with remarkable increase peptides number

Quality control or analysis of MAb biologicals

20μg

20μg5 vials

Endoproteinase Glu-C

(V8)

Cleaving at the carboxyl side of Glu and Asp. Specific at Glutamyl bond at ammonium acetatepH 4.0or ammonium bicarbonate (pH 7.8)

Proteomic research

Peptide mapping of insulin analogues

Peptide mapping of MAb

 

 

50μg

50μg5 vials

Recombinant Carboxypeptidase B

(CPB)

Preferential cleaving C-terminal basic amino acid including Lys, Arg, His

 

C-terminal sequencing

Quality control of Recombinant MAb

Removing C-terminal His-tag

20μg

20μg5 vials

Research and Analysis Use

Recombinant Enterokinase

(EK)

Specifical cleaving at the carboxyl side of Asp-Asp-Asp-Asp-Lys

Removing fusion tag of recombinant protein

Activating the enzymatic digestion of trypsinogen

50μg

50μg, 5 vials

Recombinant

Tobaccoetchvirus protease

(TEV)

Specifical cleaving at the carboxyl side of Glu-Asn-Leu-Tyr-Phe-Gln-Gly

Removing fusion tag of recombinant  protein

50μg

50μg5 vials

 

Catalogue of recombinant enzymes for industrial manufacture

Product

Character

Action Sites

Application

Specification

(lyophilized powder)

Activity

Recombinant Lysine Endopeptidase

(Lys-C)

Gene Source:

L. enzymogenes

Expression host:

E.coli

Tag-free

Cleaving at the carboxyl side  of lysine residues

Enzymatic proinsulin digestion and transpeptidation of recombinant insulin or its analoguese.g. Insulin aspart

Specific enzymatic digestion of recombinant polypeptide drugs

10mg

≥3AU/mg

1g

Recombinant

Tripsin

(TP)

Gene Source: Swine

Expression host:

P. pichia

Tag-free

Cleaving at the carboxyl side of lysine or arginine residues

Enzymatic proinsulin digestion and transpeptidation of recombinant insulin

Digestive treatment Padhereut cell cultures

Tissue enzymatic extraction

Hydrolysis of peptone preparation

10mg

≥3800USP/mg

1g

Recombinant Kexin

(Kex2)

Gene Source: Yeast

Expression host:

P. pichia

Tag-free

Cleaving at the carboxyl side of Arg-Arg or Lys-Arg

Specific enzymatic processing preparation of proinsulin glargine

Enzymatic preparation of recombinant peptide by fusion expression

10mg

≥10U/mg

1g

Carboxypeptidase B

(CPB)

Gene Source: mouse pancreas

Expression host:

P. pichia

Tag-free

Removes C-terminal Lys, Arg, His

Removing C-terminal basic amino acid of protein drug (e.g. recombinant insulin or analogues) or others

10mg

≥150U/mg

1g

Recombinant Enterokinase

(EK)

Gene Source: Swine

Expression host:

P. pichia

Tag-free

Highly recognizing sequence ‘DDDDK’ and cleaving at the lysine (K)

Used as a processing enzyme in the fusion expressing polypeptide

10mg

≥10000U/mg

1g

Recombinant

Tobaccoetchvirus protease

(TEV)

Expression host:

E. coli

With His-tag

Recognizing the carboxyl side of sequence Glu-Asn-Leu- Tyr-Phe-Gln-(Gly/Ser) and cleaving between the Gln and Gly/Ser residues.

It can be used as a processing enzyme in the fusion expression of polypeptide drugs or others

10mg

≥1000U/mg

1g

Recombinant Methionine Adenosyltransferase

(MAT)

Expression host:

E. coli

With His-tag

catalyzing the formation of S-adenosyl methionine by methionine and ATP

It can be used to produce S-adenosyl methionine

10mg

≥0.6U/mg

1g

Recombinant Arginase

Gene Source: Homo sapiens

Expression host:

E. coli

Tag-free

Converting L-arginine into L-ornithine and urea

Used to produce natural ornithine

10mg

≥20U/mg

1g

Recombinant Arginine Deiminoenzyme

Gene Source:

Mycoplasma hominis

Expression host:

E. coli

Tag-free

Catalyzing the hydrolysis of L-arginine to produce citrulline

Used to produce natural citrulline

10mg

≥30U/mg

 

1g

Recombinant 4-Hydroxyl Prolinase

(P4H)

Gene Source: ascomycetes

Expression host:

E. coli

Tag-free

Catalyzing L-proline into L-hydroxyproline

Used to produce L-hydroxyproline

Protine Hydroxylation of collagen or analogues

10mg

≥1.0U/mg

1g

Recombinant

Human Hyaluronidase

(PH20)

Gene Source:

Homo sapiens

Expression host:

CHO

Tag-free

Catalyzing the hydrolysis of large molecule hyaluronic acid

Produce low-molecular active hyaluronic acid (for cosmetics)

0.1mg

≥50, 000U/mg

0.5mg

We can provide customized package according to customers requirement

——Catalogue of recombinant enzymes for diagnosis——

Product Name

Appearance

Source

Substrate-product

Application

Activity

Urease

White lyophilized powder

Pseudomonas

UreaNH3

Enzymatic determination of urea in clinical diagnosis

 

≥100U/mg

 Sarcosine Oxidase

Yellow lyophilized powder

Bacillus sp. BSD-8

SarcosineGlycine&Formaldehyde

Enzymatic determination of creatinine, creatine, and sarcosine when coupled with creatinine amidohydrolase and creatine amidinohydrolase

8.0U/mg

Creatine Amidinohydrolase

White lyophilized powder

Pseudomonas putida

CreatineSarcosine

Enzymatic determination of creatinine when couple with creatine amidinohydrolase in clinical diagnosis

≥40U/mg

Creatinine Amidohydrolase

White lyophilized powder

Pseudomonas

CreatinineCreatine

Enzymatic determination of creatinine when coupled with creatine amidinohydrolase and sarcosine oxidase in clinical analysis

≥150U/mg

Glycerol Dehydrogenase

White lyophilized powder

Escherichia coli CFT073

GlycerolDihydroxyacetone